In Vivo Myoblasts Tracking Using the Sodium Iodide Symporter Gene Expression in Dogs.

ABSTRACT: Stem cell-based therapies are a promising approach for the treatment of degenerative muscular diseases; however, clinical trials have shown inconclusive and even disappointing results so far. Noninvasive cell monitoring by medicine imaging could improve the understanding of the survival and biodistribution of cells following injection. In this study, we assessed the canine sodium iodide symporter (cNIS) reporter gene as an imaging tool to track by single-photon emission computed tomography (SPECT/CT) transduced canine myoblasts after intramuscular (IM) administrations in dogs. cNIS-expressing cells kept their myogenic capacities and showed strong ^{99 m}Tc-pertechnetate (^{99 m}TcO₄ ^-) uptake efficiency both in vitro and in vivo. cNIS exp

SUBMITTER: Punzon I 

PROVIDER: S-EPMC7293195 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature