Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling.

ABSTRACT: Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopa

SUBMITTER: Safarian N 

PROVIDER: S-EPMC7293225 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature