Targeted mutation of secretogranin-2 disrupts sexual behavior and reproduction in zebrafish.

ABSTRACT: The luteinizing hormone surge is essential for fertility as it triggers ovulation in females and sperm release in males. We previously reported that secretoneurin-a, a neuropeptide derived from the processing of secretogranin-2a (Scg2a), stimulates luteinizing hormone release, suggesting a role in reproduction. Here we provide evidence that mutation of the scg2a and scg2b genes using TALENs in zebrafish reduces sexual behavior, ovulation, oviposition, and fertility. Large-scale spawning within-line crossings (n = 82 to 101) were conducted. Wild-type (WT) males paired with WT females successfully spawned in 62% of the breeding trials. Spawning success was reduced to 37% (P = 0.006), 44% (P = 0.0169), and 6% (P < 0.0001) for scg2a -/- , scg2b -/- , and scg2a -/- ;scg2b -/- mutants, respectiv

SUBMITTER: Mitchell K 

PROVIDER: S-EPMC7293709 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature