Pre-S Deletions are Predominant Quasispecies in HIV/HBV Infection: Quasispecies Perspective.

ABSTRACT:

Background

Combined HIV infection can accelerate HBV-induced liver disease. It is known that HBV Pre-S deletion is closely related to HBV-associated terminal liver disease in HBV mono-infection. Currently, data on HBV Pre-S quasispecies feature deletion in HIV/HBV co-infected patients are lacking.

Methods

The characteristics and blood samples of patients with chronic HBV infection were collected and classified into an HIV/HBV co-infection group and an HBV mono-infection group according to HIV antibody results before treatment. HBV DNA in serum was extracted. The HBV Pre-S region was amplified by nested-PCR and was further T-A cloned. Using the standard sequence of the matched genotype HBV as a reference, BioEdit 7.0 software was employed for sequence alignment.

Results

SUBMITTER: Nie Y 

PROVIDER: S-EPMC7294102 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature