Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases.

ABSTRACT:

Background

Esterases and lipases hydrolyze short-chain esters and long-chain triglycerides, respectively, and therefore play essential roles in the synthesis and decomposition of ester bonds in the pharmaceutical and food industries. Many SGNH family esterases share high similarity in sequences. However, they have distinct enzymatic activities toward the same substrates. Due to a lack of structural information, the detailed catalytic mechanisms of these esterases remain barely investigated.

Results

In this study, we identified two SGNH family esterases, CrmE10 and AlinE4, from marine bacteria with significantly different preferences for pH, temperature, metal ion, and organic solvent tolerance despite high sequence similarity. The crystal structures of these two esterases, i

SUBMITTER: Li Z 

PROVIDER: S-EPMC7294632 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature