Melanocortin-3 Receptors Expressed on Agouti-Related Peptide Neurons Inhibit Feeding Behavior in Female Mice.

ABSTRACT:

Objective

Activation of hypothalamic agouti-related peptide expressing (AgRP)^+ve neurons during energy deficit is a negative valence signal, rapidly activating food-seeking behaviors. This study examined the roles of melanocortin-3 receptors (MC3Rs) coexpressed in a subpopulation of AgRP^+ve neurons.

Methods

AgRP-MC3R mice expressing MC3Rs selectively in AgRP^+ve neurons were generated by crossing AgRP-IRES-Cre mice with LoxTBMc3r mice containing a "loxP-STOP-loxP" sequence in the 5' untranslated region. Body weight, body composition, and feeding behavior were assessed during ad libitum and time-restricted feeding conditions.

Results

In females, food intake of AgRP-IRES-Cre^+ve (n = 7) or AgRP-IRES-Cre^-ve (n = 9) mic

SUBMITTER: Girardet C 

PROVIDER: S-EPMC7294842 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature