Aggregation pathways of human γ D crystallin induced by metal ions revealed by time dependent methods.

ABSTRACT: Cataract formation is a slow accumulative process due to protein aggregates promoted by different factors over time. Zinc and copper ions have been reported to induce the formation of aggregates opaque to light in the human gamma D crystallin (HγD) in a concentration and temperature dependent manner. In order to gain insight into the mechanism of metal-induced aggregation of HγD under conditions that mimic more closely the slow, accumulative process of the disease, we have studied the non-equilibrium process with the minimal metal dose that triggers HγD aggregation. Using a wide variety of biophysics techniques such as turbidimetry, dynamic light scattering, fluorescence, nuclear magnetic resonance and computational methods, we obtained information on the molecular mechanisms for the forma

SUBMITTER: Fernandez-Silva A 

PROVIDER: S-EPMC7295030 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature