Quantifying E2F1 protein dynamics in single cells.

ABSTRACT:

Background

E2F1 protein, a major effector of the Rb/E2F pathway plays a central role in regulating cell-fate decisions involved in proliferation, apoptosis, and differentiation. Its expression is highly dynamic and tightly modulated through a combination of transcriptional, translational and posttranslational controls. However, the mechanisms by which its expression and activity can promote different cellular outcomes remain to be fully elucidated. To better document E2F1 expression in live cells, we have engineered a series of fluorescent E2F1 protein reporters that quantitatively capture E2F1 protein dynamics.

Methods

Reporter constructs, under the control of the mouse or human E2F1 proximal promoter, were designed to express an E2F1-Venus fusion protein incapable of bindi

SUBMITTER: Mathey-Prevot B 

PROVIDER: S-EPMC7295171 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature