Injection Molded Microfluidics for Establishing High-Density Single Cell Arrays in an Open Hydrogel Format.

ABSTRACT: Here, we develop an injection molded microfluidic approach for single cell analysis by making use of (1) rapidly curing injectable hydrogels, (2) a high density microfluidic weir trap array, and (3) reversibly bonded PDMS lids that are strong enough to withstand the injection molding process, but which can be peeled off after the hydrogel sets. This approach allows for single cell patterns to be created with densities exceeding 40 cells per mm², is amenable to high speed imaging, and creates microfluidic devices that enable efficient nutrient and gas exchange and the delivery of specific biological and chemical reagents to individual cells. We show that it is possible to organize up to 10 000 single cells in a few minutes on the device, and we developed an image analysis program

SUBMITTER: Li Y 

PROVIDER: S-EPMC7295173 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature