Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome.

ABSTRACT: A hub protein in protein interaction networks will typically have a large number of diverse interactions. Determining the core interactions and the function of such a hub protein remains a significant challenge in the study of networks. Proteins with WD40 repeats represent a large class of proteins that can be hub proteins. WDR76 is a poorly characterized WD40 repeat protein with possible involvement in DNA damage repair, cell-cycle progression, apoptosis, gene expression regulation, and protein quality control. WDR76 has a large and diverse interaction network that has made its study challenging. Here we rigorously carry out a series of affinity purification coupled to mass spectrometry (AP-MS) analyses to map out the WDR76 interactome through different biochemical conditions. We apply AP

SUBMITTER: Dayebgadoh G 

PROVIDER: S-EPMC7295179 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature