Effects of a Type I RM System on Gene Expression and Glycogen Catabolism in Synechocystis sp. PCC 6803.

ABSTRACT: Increasing evidence has shown that DNA methylation is involved in gene regulation in prokaryotes. However, there have been very limited reports about the role of DNA methylation in regulation of gene expression and physiological functions in cyanobacteria. In Synechocystis sp. PCC 6803, four genes on the plasmid pSYSX are predicted to encode the type I restriction-methylation system, slr6095 and slr6096 for the M subunit, slr6097 for the S subunit and slr6102 for the R subunit. Compared to the wild type, slr6095, slr6096, and slr6097 mutants lacked the GG^m6AN₇TTGG/CCA^m6AN₇TCC methylation in genomic DNA. Transcriptomic analysis indicated that 171 genes were reproducibly up- or down-reg

SUBMITTER: Wu D 

PROVIDER: S-EPMC7296061 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature