Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma.

ABSTRACT:

Background

LncRNAs play crucial roles in the development of carcinomas. However, the investigation of LINC00662 in Oral squamous cell carcinoma (OSCC) is still elusive.

Methods

qRT-PCR assay tested the expression levels of LINC00662, hnRNPC and AK4. With exposure to irradiation, CCK-8, colony formation, flow cytometry and western blot experiments, respectively determined the function of LINC00662 in the radiosensitivity of OSCC cells. Then RIP and western blot assays affirmed the interaction between hnRNPC protein and LINC00662 or AK4. Finally, rescue assays validated the regulation mechanism of LINC00662 in the radioresistance of OSCC.

Results

In the present report, LINC00662 was overexpressed in OSCC and its silencing could alleviate radioresistance of OSCC. Further

SUBMITTER: Chen Y 

PROVIDER: S-EPMC7296632 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature