Repeat-induced point mutation in Neurospora crassa causes the highest known mutation rate and mutational burden of any cellular life.

ABSTRACT:

Background

Repeat-induced point (RIP) mutation in Neurospora crassa degrades transposable elements by targeting repeats with C→T mutations. Whether RIP affects core genomic sequence in important ways is unknown.

Results

By parent-offspring whole genome sequencing, we estimate a mutation rate (3.38 ×  10^-6 per bp per generation) that is two orders of magnitude higher than reported for any non-viral organism, with 93-98% of mutations being RIP-associated. RIP mutations are, however, relatively rare in coding sequence, in part because RIP preferentially attacks GC-poor long duplicates that interact in three dimensional space, while coding sequence duplicates are rare, GC-rich, short, and tend not to interact. Despite this, with over 5 coding sequence mutations per ge

SUBMITTER: Wang L 

PROVIDER: S-EPMC7296669 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature