A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease.

ABSTRACT:

Background

Kawasaki disease (KD) is the leading cause of childhood acquired heart disease in developed nations and can result in coronary artery aneurysms and death. Clinical and epidemiologic features implicate an infectious cause but specific antigenic targets of the disease are unknown. Peripheral blood plasmablasts are normally highly clonally diverse but the antibodies they encode are approximately 70% antigen-specific 1-2 weeks after infection.

Methods

We isolated single peripheral blood plasmablasts from children with KD 1-3 weeks after onset and prepared 60 monoclonal antibodies (mAbs). We used the mAbs to identify their target antigens and assessed serologic response among KD patients and controls to specific antigen.

Results

Thirty-two mAbs from 9 of 11 pati

SUBMITTER: Rowley AH 

PROVIDER: S-EPMC7296860 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature