Activity-based protein profiling guided identification of urine proteinase 3 activity in subclinical rejection after renal transplantation.

ABSTRACT:

Background

The pathophysiology of subclinical versus clinical rejection remains incompletely understood given their equivalent histological severity but discordant graft function. The goal was to evaluate serine hydrolase enzyme activities to explore if there were any underlying differences in activities during subclinical versus clinical rejection.

Methods

Serine hydrolase activity-based protein profiling (ABPP) was performed on the urines of a case control cohort of patients with biopsy confirmed subclinical or clinical transplant rejection. In-gel analysis and affinity purification with mass spectrometry were used to demonstrate and identify active serine hydrolase activity. An assay for proteinase 3 (PR3/PRTN3) was adapted for the quantitation of activity in urine.

Re

SUBMITTER: Navarrete M 

PROVIDER: S-EPMC7296916 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature