SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of β-catenin.

ABSTRACT:

Background & aims

The canonical Wnt signaling pathway activates the transcriptional activity of β-catenin. This pathway is often activated in colorectal cancer cells, but strategies to block it in tumors have not been effective. The SAM pointed domain containing ETS transcription factor (SPDEF) suppresses formation of colon tumors by unclear mechanisms. We investigated these mechanisms and the effects of SPDEF on β-catenin activity in mouse models of colorectal cancer (CRC), CRC cell lines, and mouse and human normal and cancer colonoids.

Methods

We performed studies of Lgr5^CreERT2; β-catenin^exon3; Rosa26^{LSL-rtta-ires-EGFP}; TRE-Spdef mice, which express an oncogenic form of β-catenin in Lgr5-positive ISCs upon administration of tamoxifen and

SUBMITTER: Lo YH 

PROVIDER: S-EPMC7297058 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature