Lipidomics of human adipose tissue reveals diversity between body areas.

ABSTRACT:

Background and aims

Adipose tissue plays a pivotal role in storing excess fat and its composition reflects the history of person's lifestyle and metabolic health. Broad profiling of lipids with mass spectrometry has potential for uncovering new knowledge on the pathology of obesity, metabolic syndrome, diabetes and other related conditions. Here, we developed a lipidomic method for analyzing human subcutaneous adipose biopsies. We applied the method to four body areas to understand the differences in lipid composition between these areas.

Materials and methods

Adipose tissue biopsies from 10 participants were analyzed using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. The sample preparation optimization included the opt

SUBMITTER: Al-Sari N 

PROVIDER: S-EPMC7297320 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature