Discriminating between negative cooperativity and ligand binding to independent sites using pre-equilibrium properties of binding curves.

ABSTRACT: Negative cooperativity is a phenomenon in which the binding of a first ligand or substrate molecule decreases the rate of subsequent binding. This definition is not exclusive to ligand-receptor binding, it holds whenever two or more molecules undergo two successive binding events. Negative cooperativity turns the binding curve more graded and cannot be distinguished from two independent and different binding events based on equilibrium measurements only. The need of kinetic data for this purpose was already reported. Here, we study the binding response as a function of the amount of ligand, at different times, from very early times since ligand is added and until equilibrium is reached. Over those binding curves measured at different times, we compute the dynamic range: the fold change req

SUBMITTER: Sevlever F 

PROVIDER: S-EPMC7297384 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature