Three-dimensional real time imaging of amyloid β aggregation on living cells.

ABSTRACT: Alzheimer's disease (AD) is a progressive disorder of the brain that gradually decreases thinking, memory, and language abilities. The aggregation process of amyloid β (Aβ) is a key step in the expression of its neurocytotoxicity and development of AD because Aβ aggregation and accumulation around neuronal cells induces cell death. However, the molecular mechanism underlying the neurocytotoxicity and cell death by Aβ aggregation has not been clearly elucidated. In this study, we successfully visualized real-time process of Aβ₄₂ aggregation around living cells by applying our established QD imaging method. 3D observations using confocal laser microscopy revealed that Aβ₄₂ preferentially started to aggregate at the region where membrane protrusions frequently formed. Fu

SUBMITTER: Kuragano M 

PROVIDER: S-EPMC7297742 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature