Alcalase Microarray Base on Metal Ion Modified Hollow Mesoporous Silica Spheres as a Sustainable and Efficient Catalysis Platform for Proteolysis.

ABSTRACT: The industrial exploitation of protease is limited owing to its sensitivity to environmental factors and autolysis during biocatalytic processes. In the present study, the alcalase microarray (Bacillus licheniformis, alcalase@HMSS-NH₂-Metal) based on different metal ions modified hollow mesoporous silica spheres (HMSS-NH₂-Metal) was successfully developed via a facile approach. Among the alcalase@HMSS-NH₂-Metal (Ca²⁺, Zn²⁺, Fe³⁺, Cu²⁺), the alcalase@HMSS-NH₂-Fe³⁺ revealed the best immobilization efficiency and enzymatic properties. This tailor-made nanocomposite immobilized alcalase on a surface-bound network of amino-metal complex bearing protein-modifiable sites via met

SUBMITTER: Zeng Q 

PROVIDER: S-EPMC7297948 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature