Identification and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium officinale.

ABSTRACT: Dendrobium officinale Kimura et Migo is a famous precious medicinal plant in China. Seed and seedling were cultivated with the mycorrhizal fungus Sebacina sp. CCaMK was initially cloned from D. officinale based on a SSH cDNA library of symbiotically germinated seeds with Sebacina sp. Phylogenetic analysis was performed among DoCCaMK and other CCaMKs. The particle bombardment technique was used to visualize DoCCaMK-GFP. qRT-PCR and western blot analysis were conducted to determine the tissue expression patterns of DoCCaMK with (SGS) and without (UGS) Sebacina sp. Furthermore, the effect of KN-93 on CCaMK expression was also examined. Using NMT the net Ca²⁺ fluxes and the CCaMK concentration were measured during D. officinale seed germination. DoCCaMK had the highest homology with

SUBMITTER: Xing YM 

PROVIDER: S-EPMC7298032 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature