High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes.

ABSTRACT: Plasmodium falciparum is the causative agent of the deadliest human malaria. New molecules are needed that can specifically bind to erythrocytes that are infected with P. falciparum for diagnostic purposes, to disrupt host-parasite interactions, or to deliver chemotherapeutics. Aptamer technology has the potential to revolutionize biological diagnostics and therapeutics; however, broad adoption is hindered by the high failure rate of the systematic evolution of ligands by exponential enrichment (SELEX). Here we performed parallel SELEX experiments to compare the impact of two different methods for single-strand recovery on the efficiency of aptamer enrichment. Our experimental results and analysis of SELEX publications spanning 13 years implicate the alkaline denaturation step as a signifi

SUBMITTER: Oteng EK 

PROVIDER: S-EPMC7298056 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature