A prospective longitudinal study on the microbiota composition in amyotrophic lateral sclerosis.

ABSTRACT:

Background

A connection between amyotrophic lateral sclerosis (ALS) and altered gut microbiota composition has previously been reported in animal models. This work is the first prospective longitudinal study addressing the microbiota composition in ALS patients and the impact of a probiotic supplementation on the gut microbiota and disease progression.

Methods

Fifty patients and 50 matched controls were enrolled. The microbial profile of stool samples from patients and controls was analyzed via PCR-Denaturing Gradient Gel Electrophoresis, and the main microbial groups quantified via qPCR. The whole microbiota was then analyzed via next generation sequencing after amplification of the V3-V4 region of 16S rDNA. Patients were then randomized to receive probiotic treatment or pl

SUBMITTER: Di Gioia D 

PROVIDER: S-EPMC7298784 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature