25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner.

ABSTRACT:

Background

Genome-wide association studies of Alzheimer's disease (AD) have implicated pathways related to lipid homeostasis and innate immunity in AD pathophysiology. However, the exact cellular and chemical mediators of neuroinflammation in AD remain poorly understood. The oxysterol 25-hydroxycholesterol (25-HC) is an important immunomodulator produced by peripheral macrophages with wide-ranging effects on cell signaling and innate immunity. Cholesterol 25-hydroxylase (CH25H), the enzyme responsible for 25-HC production, has also been found to be one of the disease-associated microglial (DAM) genes that are upregulated in the brain of AD and AD transgenic mouse models.

Methods

We used real-time PCR and immunoblotting to examine CH25H expression in human AD brain tissue and

SUBMITTER: Wong MY 

PROVIDER: S-EPMC7298825 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature