Follicular B2 Cell Activation and Class Switch Recombination Depend on Autocrine C3ar1/C5ar1 Signaling in B2 Cells.

ABSTRACT: The involvement of complement in B2 cell responses has been regarded as occurring strictly via complement components in plasma. In this study, we show that Ab production and class switch recombination (CSR) depend on autocrine C3a and C5a receptor (C3ar1/C5ar1) signaling in B2 cells. CD40 upregulation, IL-6 production, growth in response to BAFF or APRIL, and AID/Bcl-6 expression, as well as follicular CD4⁺ cell CD21 production, all depended on this signal transduction. OVA immunization of C3ar1^-/-C5ar1^-/- mice elicited IgM Ab but no other isotypes, whereas decay accelerating factor (Daf1)^-/- mice elicited more robust Ab production and CSR than wild-type (WT) mice. Comparable differences occurred in OVA-immunized μMT recipien

SUBMITTER: Paiano J 

PROVIDER: S-EPMC7299189 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature