Energetic determinants of protein binding specificity: insights into protein interaction networks.

ABSTRACT: One of the challenges of the postgenomic era is to provide a more realistic representation of cellular processes by combining a systems biology description of functional networks with information on their interacting components. Here we carried out a systematic large-scale computational study on a structural protein-protein interaction network dataset in order to dissect thermodynamic characteristics of binding determining the interplay between protein affinity and specificity. As expected, interactions involving specific binding sites display higher affinities than those of promiscuous binding sites. Next, in order to investigate a possible role of modular distribution of hot spots in binding specificity, we divided binding sites into modules previously shown to be energetically independe

SUBMITTER: Carbonell P 

PROVIDER: S-EPMC7299235 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature