An ECF-type transporter scavenges heme to overcome iron-limitation in Staphylococcus lugdunensis.

ABSTRACT: Energy-coupling factor type transporters (ECF) represent trace nutrient acquisition systems. Substrate binding components of ECF-transporters are membrane proteins with extraordinary affinity, allowing them to scavenge trace amounts of ligand. A number of molecules have been described as substrates of ECF-transporters, but an involvement in iron-acquisition is unknown. Host-induced iron limitation during infection represents an effective mechanism to limit bacterial proliferation. We identified the iron-regulated ECF-transporter Lha in the opportunistic bacterial pathogen Staphylococcus lugdunensis and show that the transporter is specific for heme. The recombinant substrate-specific subunit LhaS accepted heme from diverse host-derived hemoproteins. Using isogenic mutants and recombinant e

SUBMITTER: Jochim A 

PROVIDER: S-EPMC7299338 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature