A regulatory microRNA network controls endothelial cell phenotypic switch during sprouting angiogenesis.

ABSTRACT: Angiogenesis requires the temporal coordination of the proliferation and the migration of endothelial cells. Here, we investigated the regulatory role of microRNAs (miRNAs) in harmonizing angiogenesis processes in a three-dimensional in vitro model. We described a microRNA network which contributes to the observed down- and upregulation of proliferative and migratory genes, respectively. Global analysis of miRNA-target gene interactions identified two sub-network modules, the first organized in upregulated miRNAs connected with downregulated target genes and the second with opposite features. miR-424-5p and miR-29a-3p were selected for the network validation. Gain- and loss-of-function approaches targeting these microRNAs impaired angiogenesis, suggesting that these modules are instrumenta

SUBMITTER: Rosano S 

PROVIDER: S-EPMC7299339 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature