GTP hydrolysis by Synechocystis IM30 does not decisively affect its membrane remodeling activity.

ABSTRACT: The function of IM30 (also known as Vipp1) is linked to protection and/or remodeling of the thylakoid membrane system in chloroplasts and cyanobacteria. Recently, it has been revealed that the Arabidopsis IM30 protein exhibits GTP hydrolyzing activity in vitro, which was unexpected, as IM30 does not show any classical GTPase features. In the present study, we addressed the question, whether an apparent GTPase activity is conserved in IM30 proteins and can also be observed for IM30 of the cyanobacterium Synechocystis sp. PCC 6803. We show that Synechocystis IM30 is indeed able to bind and hydrolyze GTP followed by the release of P_i. Yet, the apparent GTPase activity of Synechocystis IM30 does not depend on Mg²⁺, which, together with the lack of classical GTPase feature

SUBMITTER: Junglas B 

PROVIDER: S-EPMC7299955 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature