Characterization of red blood cell microcirculatory parameters using a bioimpedance microfluidic device.

ABSTRACT: This paper describes the use of a microfluidic device comprising channels with dimensions mimicking those of the smallest capillaries found in the human microcirculation. The device structure, associated with a pair of microelectrodes, provides a tool to electrically measure the transit time of red blood cells through fine capillaries and thus generate an electrical signature for red blood cells in the context of human erythroid genetic disorders, such as sickle cell disease or hereditary spherocytosis, in which red cell elasticity is altered. Red blood cells from healthy individuals, heated or not, and red blood cells from patients with sickle cell disease or hereditary spherocytosis where characterized at a single cell level using our device. Transit time and blockade amplitude recording

SUBMITTER: Xu T 

PROVIDER: S-EPMC7299978 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature