Ontology highlight
ABSTRACT:
SUBMITTER: Blin K
PROVIDER: S-EPMC7301206 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Synthetic and systems biotechnology 20200613 2
CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these issues. The recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base exchange. These base editing systems however require additional ...[more]