Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin.

ABSTRACT:

Background

Immunotoxin is a hybrid protein consisting of a toxin moiety that is linked to a targeting moiety for the purpose of specific elimination of target cells. Toxins used in traditional immunotoxins are practically difficult to be produced in large amount, have poor tissue penetration and a complex internalization process. We hypothesized that the smaller HALT-1, a cytolysin derived from Hydra magnipapillata, can be used as the toxin moiety in construction of a recombinant immunotoxin.

Results

In this study, pro-inflammatory macrophage was selected as the target cell due to its major roles in numerous inflammatory and autoimmune disorders. We aimed to construct macrophage-targeted recombinant immunotoxins by combining HALT-1 with anti-CD64-scFv in two orientations, an

SUBMITTER: Jiemy WF 

PROVIDER: S-EPMC7301450 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature