Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system.

ABSTRACT:

Background

Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice.

Results

The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expr

SUBMITTER: Chang D 

PROVIDER: S-EPMC7301529 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature