Novel Applications of Biocatalysis to Stereochemistry Determination of 2'3'-cGAMP Bisphosphorothioate (2'3'-cG^SA^SMP).

ABSTRACT: The metazoan second messenger 2'3'-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) is a cyclic dinucleotide (CDN) that induces secretion of type I interferons and activates the immune system and has thus attracted significant interest as a vaccine adjuvant or immunotherapeutic. CDN bisphosphorothioates are of particular interest because of their increased hydrolytic stability and improved cell activities. In our work with CDN bisphosphorothioates, we sought a method for systematic determination of the absolute stereochemistry of their phosphorothioate stereocenters. A novel biocatalytic method employing snake venom phosphodiesterase (svPDE) and nP1 has been developed and successfully applied to stereochemistry determination of 2'3'-cGAMP bisphosphorothioates. This method una

SUBMITTER: Lim J 

PROVIDER: S-EPMC7301576 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature