An intrinsic, label-free signal for identifying stem cell-derived cardiomyocyte subtype.

ABSTRACT: Human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes have many promising applications, including the regeneration of injured heart muscles, cardiovascular disease modeling, and drug cardiotoxicity screening. Current differentiation protocols yield a heterogeneous cell population that includes pluripotent stem cells and different cardiac subtypes (pacemaking and contractile cells). The ability to purify these cells and obtain well-defined, controlled cell compositions is important for many downstream applications; however, there is currently no established and reliable method to identify hiPSC-derived cardiomyocytes and their subtypes. Here, we demonstrate that second harmonic generation (SHG) signals generated directly from the myosin rod bundles can be a label-free, intrinsi

SUBMITTER: Chang CW 

PROVIDER: S-EPMC7302059 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature