Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly.

ABSTRACT: The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of the protein building blocks and clathrin self-assemblies to coat liposomes with biomaterials, advanced hybrid carriers can be derived. Here, we present a method for fabricating DNA-coated liposomes by hydrophobically anchoring and subsequently connecting DNA-based triskelion structures on the liposome surface inspired by the assembly of the protein clathrin. Dynamic light scattering, ζ-potential, confocal microscopy, and cryo-electron microscopy measurements independently demonstrate successful DNA coating. Nanomechanical measurements conduc

SUBMITTER: Baumann KN 

PROVIDER: S-EPMC7302506 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature