Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis.

ABSTRACT: New techniques for obtaining electron microscopy data through the cell volume are being increasingly utilized to answer cell biologic questions. Here, we present a three-dimensional atlas of Plasmodium falciparum ultrastructure throughout parasite cell division. Multiple wild type schizonts at different stages of segmentation, or budding, were imaged and rendered, and the 3D structure of their organelles and daughter cells are shown. Our high-resolution volume electron microscopy both confirms previously described features in 3D and adds new layers to our understanding of Plasmodium nuclear division. Interestingly, we demonstrate asynchrony of the final nuclear division, a process that had previously been reported as synchronous. Use of volume electron microscopy techniques for biological

SUBMITTER: Rudlaff RM 

PROVIDER: S-EPMC7302870 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature