Transcriptional profiling and immunophenotyping show sustained activation of blood monocytes in subpatent Plasmodium falciparum infection.

ABSTRACT:

Objectives

Malaria, caused by Plasmodium infection, remains a major global health problem. Monocytes are integral to the immune response, yet their transcriptional and functional responses in primary Plasmodium falciparum infection and in clinical malaria are poorly understood.

Methods

The transcriptional and functional profiles of monocytes were examined in controlled human malaria infection with P. falciparum blood stages and in children and adults with acute malaria. Monocyte gene expression and functional phenotypes were examined by RNA sequencing and flow cytometry at peak infection and compared to pre-infection or at convalescence in acute malaria.

Results

In subpatent primary infection, the monocyte transcriptional profile was dominated by

SUBMITTER: Loughland JR 

PROVIDER: S-EPMC7302943 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature