Adenosine-producing regulatory B cells in head and neck cancer.

ABSTRACT:

Background

Multiple mechanisms of immunosuppression have been identified in the tumor microenvironment including regulatory B cells (B_reg). Recently, we have shown that B_reg suppress T cell function by production of adenosine (ADO). However, the autocrine effect of ADO on B cells and the role of B_reg in head and neck cancer remains unclear.

Methods

Blood (n = 42) and tumor tissue (n = 39) of head and neck cancer patients and healthy donors (n = 60) were analyzed by FACS. The effect of ADO on phenotype, intracellular signaling pathways, Ca²⁺ influx and ADO production was analyzed in B_reg and effector B cells (B_eff) by FACS, luminescence and mass spectrometry. The blockage of the ADO receptor A_2A was

SUBMITTER: Jeske SS 

PROVIDER: S-EPMC7303082 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature