Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.

ABSTRACT: Core Binding Factors (CBFs) are a small group of heterodimeric transcription factor complexes composed of DNA binding proteins, RUNXs, and a non-DNA binding protein, CBFB. The LH surge increases the expression of Runx1 and Runx2 in ovulatory follicles, while Cbfb is constitutively expressed. To investigate the physiological significance of CBFs, we generated a conditional mutant mouse model in which granulosa cell expression of Runx2 and Cbfb was deleted by the Esr2Cre. Female Cbfb^flox/flox;Esr2^cre/+;Runx2^flox/flox mice were infertile; follicles developed to the preovulatory follicle stage but failed to ovulate. RNA-seq analysis of mutant mouse ovaries collected at 11 h post-hCG unveiled numerous CBFs-downstream genes that are associated with inflammation,

SUBMITTER: Lee-Thacker S 

PROVIDER: S-EPMC7303197 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature