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Sequence analysis and protein interactions of Arabidopsis CIA2 and CIL proteins.


ABSTRACT:

Background

A previous screening of Arabidopsis thaliana for mutants exhibiting dysfunctional chloroplast protein transport identified the chloroplast import apparatus (cia) gene. The cia2 mutant has a pale green phenotype and reduced rate of protein import into chloroplasts, but leaf shape and size are similar to wild-type plants of the same developmental stage. Microarray analysis showed that nuclear CIA2 protein enhances expression of the Toc75, Toc33, CPN10 and cpRPs genes, thereby up-regulating protein import and synthesis efficiency in chloroplasts. CIA2-like (CIL) shares 65% sequence identity to CIA2, suggesting that CIL and CIA2 are homologous proteins in Arabidopsis. Here, we further assess the protein interactions and sequence features of CIA2 and CIL.

Results

Subce

SUBMITTER: Yang CY 

PROVIDER: S-EPMC7303255 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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