Regulation of R1 Plasmid Transfer by H-NS, ArcA, TraJ, and DNA Sequence Elements.

ABSTRACT: In conjugative elements such as integrating conjugative elements (ICEs) or conjugative plasmids (CPs) transcription of DNA transfer genes is a prerequisite for cells to become transfer competent, i.e., capable of delivering plasmid DNA via bacterial conjugation into new host bacteria. In the large family of F-like plasmids belonging to the MobF₁₂A group, transcription of DNA transfer genes is tightly controlled and dependent on the activation of a single promoter, designated P_Y. Plasmid encoded TraJ and chromosomally encoded ArcA proteins are known activators, whereas the nucleoid associated protein heat-stable nucleoid structuring (H-NS) silences the P_Y promoter. To better understand the role of these proteins in P_Y promoter activation, we perfo

SUBMITTER: Bischof K 

PROVIDER: S-EPMC7303359 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature