A novel immunofluorescence detection method for renal cell-type specific in situ cytokine production by confocal microscopy.

ABSTRACT: The detection of cytokines production in tissues is subjected to significant limitations: (1) Cytokine protein production frequently does not correlate with mRNA levels. (2) Cytokines are secreted rapidly and dissipate from the cellular source, thus making detection difficult. (3) The synthetic rate of many cytokines are low. (4) Tissue fixation ablates antigenic sites and diminishes detection signals. The identification of the cellular sources of cytokines poses an additional challenge because of the lack of suitable and readily available cellular markers. In our renal cytokine production studies in lupus nephritis, we have established methods to resolve problems associated with the identification of cellular sources of pertinent cytokines in the glomerulus and interstitium. Four-color co

SUBMITTER: Sung SJ 

PROVIDER: S-EPMC7303990 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature