Reversal of glucocorticoid resistance in Acute Lymphoblastic Leukemia cells by miR-145.

ABSTRACT:

Objective

To analyze the expression levels of miR-145 in ALL children and their effects on the prognosis of ALL and to explore the mechanism of miR-145 in reversing the resistance of ALL cells to glucocorticoids.

Methods

A GEO database dataset was used to analyze the expression levels of miR-145 in ALL children. The association between miR-145 and childhood prognosis was analyzed by the TARGET database data. The expression levels of miR-145 in the glucocorticoid-resistant ALL cell line CEM-C1 were increased by lipofectamine 2000-mediated transfection. Cell proliferation inhibition experiments were performed to detect the effect of miR-145 on the response of CEM-C1 cell line to glucocorticoids. The expression levels of the apoptotic, autophagic and drug resistance-associated

SUBMITTER: Long S 

PROVIDER: S-EPMC7304417 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature