Excess nitrate induces nodule greening and reduces transcript and protein expression levels of soybean leghaemoglobins.

ABSTRACT:

Background and aims

Efficient biological nitrogen fixation (BNF) requires leghaemoglobin (Lb) to modulate oxygen pressure in nodules. Excess N supply severely inhibits BNF through effects on Lb during nodulation. As yet, a systematic identification and characterization of Lb-encoding genes in soybean has not been reported.

Methods

The effects of N on BNF were studied in soybean plants inoculated with rhizobia and exposed to excess or low N availability in hydroponic cultures. To identify soybean Lb proteins, BLAST searches were performed on the Phytozome website. Bioinformatic analysis of identified GmLbs was then carried out to investigate gene structure, protein homology and phylogenetic relationships. Finally, quantitative real-time PCR was employed to analyse the express

SUBMITTER: Du M 

PROVIDER: S-EPMC7304466 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature