The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes.

ABSTRACT: Recent applications of mass spectrometry (MS) to study membrane protein complexes are yielding valuable insights into the binding of lipids and their structural and functional roles. To date, most native MS experiments with membrane proteins are based on detergent solubilization. Many insights into the structure and function of membrane proteins have been obtained using detergents; however, these can promote local lipid rearrangement and can cause fluctuations in the oligomeric state of protein complexes. To overcome these problems, we developed a method that does not use detergents or other chemicals. Here we report a detailed protocol that enables direct ejection of protein complexes from membranes for analysis by native MS. Briefly, lipid vesicles are prepared directly from membranes of

SUBMITTER: Chorev DS 

PROVIDER: S-EPMC7305028 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature