HFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.

ABSTRACT: Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.

SUBMITTER: Hildebrand S 

PROVIDER: S-EPMC7305111 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature