Diverse proteins aggregate in mild cognitive impairment and Alzheimer's disease brain.

ABSTRACT:

Background

All cells accumulate insoluble protein aggregates throughout their lifespan. While many studies have characterized the canonical disease-associated protein aggregates, such as those associated with amyloid plaques, additional, undefined proteins aggregate in the brain and may be directly associated with disease and lifespan.

Methods

A proteomics approach was used to identify a large subset of insoluble proteins in the mild cognitively impaired (MCI) and Alzheimer's disease (AD) human brain. Cortical samples from control, MCI, and AD patients were separated into detergent-soluble and detergent-insoluble fractions, and high-resolution LC/MS/MS technology was used to determine which proteins became more insoluble in the disease state. Bioinformatics analyses were use

SUBMITTER: Kepchia D 

PROVIDER: S-EPMC7305608 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature