CRISPR screen in regulatory T cells reveals modulators of Foxp3.

ABSTRACT: Regulatory T (T_reg) cells are required to control immune responses and maintain homeostasis, but are a significant barrier to antitumour immunity¹. Conversely, T_reg instability, characterized by loss of the master transcription factor Foxp3 and acquisition of proinflammatory properties², can promote autoimmunity and/or facilitate more effective tumour immunity^3,4. A comprehensive understanding of the pathways that regulate Foxp3 could lead to more effective T_reg therapies for autoimmune disease and cancer. The availability of new functional genetic tools has enabled the possibility of systematic dissection of the gene regulatory programs that modulate Foxp3 expression. Here we developed a CRISPR-based pooled screening platfo

SUBMITTER: Cortez JT 

PROVIDER: S-EPMC7305989 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature