Enhancement of gibberellic acid production from Fusarium fujikuroi by mutation breeding and glycerol addition.

ABSTRACT: Gibberellic acid (GA₃) is a natural plant growth hormone that has been widely used in agriculture and horticulture. To obtain higher GA₃ producing strains, the method of screening the strains for resistance to simvastatin was used after treatment with nitrosoguanidine (NTG) and gamma rays. The rationale for the strategy was that mutants showing simvastatin resistance were likely to be high GA₃ producers, as their activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is relatively more effective. GA₃ yield of mutant S109 increased by 14.2% than that of the original strain. The GA₃ production ability in mutant S109 remained relatively stable after ten generations. With the addition of 0.4 g glycerol on the 5th day during

SUBMITTER: Peng XL 

PROVIDER: S-EPMC7306055 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature